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Bioluminescence is one of the greatest wonders of nature. Likewise, mushrooms are magnificent living beings that present structures with very whimsical and beautiful shapes. Thus, bioluminescent mushrooms are a work of art. However, science has become interested in them not only for their aesthetics, but also for their possible applications. From using some of their elements to track some cells to creating plants that emit light, bioluminescent fungi have been used by biotechnology to achieve things worthy of a science fiction movie. The purpose of this popular science article is to address topics such as what is bioluminescence, what are bioluminescent fungi, their origin, where they are found, how their bioluminescence is produced, what is their use, their ecological role and some of the applications they have had in biotechnology.